RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
نویسندگان
چکیده
منابع مشابه
Laboratory intercomparison of the cytokinesis-block micronucleus assay.
The focus of the study is an intercomparison of laboratories' dose-assessment performances using the cytokinesis-block micronucleus (CBMN) assay as a diagnostic triage tool for individual radiation dose assessment. Homogenously X-irradiated (240 kVp, 1 Gy/min) blood samples for establishing calibration data (0.25-5 Gy) as well as blind samples (0.1-6.4 Gy) were sent to the participants. The CBM...
متن کاملTesting chemical agents with the cytokinesis-block micronucleus cytome assay.
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimeti...
متن کاملMicronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay
This study evaluated the clastogenic and/or aneugenic potential of three nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte cultures. All three inhibitors produced a positive response when tested in binucleated cells. The genotoxicity of AZT and 3TC was restricted to binuc...
متن کاملGenotoxicity induced by arsenic compounds in peripheral human lymphocytes analysed by cytokinesis-block micronucleus assay.
This work focuses on the analysis of genotoxic effects on human peripheral lymphocytes exposed in vitro to different arsenic (As) compounds by means of the cytokinesis-block micronucleus assay. The study was carried out by challenging peripheral human lymphocytes with six As compounds in trivalent or pentavalent forms such as arsenite (As(III)) and arsenate (As(V)) and organoarsenic species suc...
متن کاملMicronucleus assay of human lymphocytes: a comparison of cytokinesis-block and human capillary blood lymphocytes methods.
Micronucleus (MN) expression in Peripheral blood lymphocytes is well established as a method to monitor chromosome damage in human populations. The use of MN as a measure of chromosome damage in peripheral blood lymphocytes was first proposed by Countryman and Heddle and subsequently improved with the development of the cytokinesis-block micronucleus (CBMN) method, which has been extensively us...
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ژورنال
عنوان ژورنال: International Journal of Radiation Biology
سال: 2016
ISSN: 0955-3002,1362-3095
DOI: 10.1080/09553002.2016.1206231